top of page
MS_5_edited.jpg

QconCAT Technology

QconCATs (Quantification conCATamer) are custom-made synthetic proteins, usually comprising of up to 50 heavy isotope-labelled proteotypic peptides, functioning as true internal standards for absolute protein quantification using mass spectrometry.

Original publications:

  • Beynon R. J. et al., Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides. Nature Methods Vol. 2 (8), August 2005, 587-589.

  • Pratt J. M. et al., Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes. Nature Protocols Vol. 1 (2), 2006, 1029-1043.

Technology

Application areas

References

  • Full humanization of the glycolytic pathway in Saccharomyces cerevisiae
    Boonekamp FJ, Knibbe E, Vieira-Lara MA, Wijsman M, Luttik MAH, van Eunen K, Ridder MD, Bron R, Almonacid Suarez AM, van Rijn P, Wolters JC, Pabst M, Daran JM, Bakker BM, Daran-Lapujade P.
    Cell Rep. 2022 Jun 28;39(13):111010. [Pubmed]
    Boonekamp et al. generated and characterized a yeast strain with a humanized glycolytic pathway, providing a new model to study human glycolysis in a simplified context. In their study they used 6 QconCATs to determine absolute concentrations of glycolytic targets by targeted proteomics.

  • Absolute protein quantification of the yeast chaperome under conditions of heat shock
    Mackenzie RJ, Lawless C, Holman SW, Lanthaler K, Beynon RJ, Grant CM, Hubbard SJ, Eyers CE.
    Proteomics. 2016 Jun 2. [PubMed]
    Mackenzie et al. determined copy per cell values for 49 key chaperones in S. cerevisiae under conditions of normal growth and heat shock, by selected reaction monitoring (SRM) mass spectrometry using QconCATs as reference peptides.

  • Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring
    Lawless C, Holman SW, Brownridge P, Lanthaler K, Harman VM, Watkins R, Hammond DE, Miller RL, Sims PF, Grant CM, Eyers CE, Beynon RJ, Hubbard SJ.
    Mol Cell Proteomics. 2016 Apr;15(4):1309-22. [PubMed]
    Lawless et al. quantified over 1800 S. cerevisiae proteins by selected reaction monitoring (SRM) mass spectrometry using over 100 QconCATs.

bottom of page