Cov-MS peptide standards
Cov-MS
Peptide reference standard for detection of SARS-CoV-2 using mass spectrometry
Bioinformatic support for data analysis is available upon request.
-
Detect and quantify SARS-CoV-2 in e.g. nasopharyngeal swab samples
-
Validated reference peptides for SARS-CoV-2 NCAP and spike protein
-
Additional peptides for SARS-CoV, Coronaviridae and their lowest common ancestor
-
Control peptides for sample quality and synthetic peptides for workflow calibration and standardization
-
Based on QconCAT technology
The Cov-MS peptide standard for mass spectrometry detection and absolute quantification of SARS-CoV-2 proteins was co-developed by PolyQuant and University of Ghent (Van Puyvelde et al., 2020: Cov-MS: a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients).
Available Products
References
Cov-MS: a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients
Van Puyvelde B, et al.,
JACS Au 2021 Jun 28;1(6):750-765. [PubMed]
In a consortium consisting of 15 academic laboratories and several industrial partners, Van Puyvelde et al., developed an alternative diagnostic test that detects proteolytically digested SARS-CoV-2 proteins using mass spectrometry (MS). Their highly sensitivetest provides an orthogonal and complementary approach to RT-PCR,
Cov2MS: An Automated and Quantitative Matrix-Independent Assay for Mass Spectrometric Measurement of SARS-CoV-2 Nucleocapsid Protein
van Puyvelde B, et al.,
Anal. Chem. 2022, 94, 50, 17379–17387 [PubMed]
Van Puyvelde et al., advance their Cov-MS test by adding of SISCAPA technology to enrich proteotypic peptides of the SARS-CoV-2 nucleocapsid (N) protein from trypsin-digested patient samples (nasopharyngeal swabs, saliva, and plasma) and has increased sensitivity (comparable to qPCR cycle of 30-31).
A fast and sensitive absolute quantification assay for the detection of SARS-CoV-2 peptides using parallel reaction monitoring mass spectrometry
Gajbhiye A et al.,
J Proteomics. 2022 Aug 15;265:104664. [PubMed]
Gajbhiye et al., developed a high-throughput and sensitive PRM-MS assay for absolute quantification of SARS-CoV-2 nucleocapsid peptides with short turn-around times using Cov-MS as reference standard.
Cov-MS-2
Peptide reference standard for detection of viruses infecting the respiratory tract using mass spectrometry
-
Parallel detection and quantification of SARS-CoV-2, Influenza A, Influenza B and RSV.
-
Synthetic peptides for workflow calibration and standardization.
-
Based on QconCAT technology.
The Cov-MS-2 peptide standard for mass spectrometry detection and absolute quantification of SARS-CoV-2, Influenza and RSV proteins was co-developed by PolyQuant and the University of Ghent.
Bioinformatic support for data analysis is available upon request.
