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Whitepaparer & Application notes
Quality control of recombinant proteins and medical products using LC-MS/MS – Quantification of Host Cell Proteins
Our Application Note describes how we use LC-MS/MS for unbiased detection of HCPs but also for targeted detection of the most critical HCPs and for quantification of the contaminations using reference standards like QconCATs.
Intra-sample three-point quantitative calibration
We show the advantages and drawbacks of metabolic QconCAT labelling using 15N salt and/or 13C glucose compared to classical SILAC labelling. By spiking multiple differently labelled QconCATs at increasing concentrations into the same sample, we establish an intra-sample three-point calibration curve.
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