EcoCAT - Host cell protein analysis
EcoCAT
Targeted measurement and absolute quantification of E. coli-derived host cell proteins
Biopharmaceutical drugs involving recombinant proteins produced in E. coli, require identification and risk analysis of all residual host cell derived contaminations (host cell proteins/HCP).
PolyQuant’s EcoCAT Kit provides 100 heavy isotope (15N) labelled reference peptides for absolute protein quantification of 48 common and critical E. coli-specific host cell proteins (HCP) using LC-MS/MS in nanoFlow.​​
Our technology is compatible with various sample preparation protocols (e. g. in-solution, FASP, SP3) and different machine methods (e.g. DIA/SWATH, PRM/MRM, SureQuant) and enables easy setup of multiprotein methods.
Other labeling options and bioinformatic support are available upon request. For package sizes suitable for microFlow, please contact us.
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PolyQuant has established routine protocols for targeted LC-MS/MS measurement of the kit’s peptides. Contact us for sample preparation and measurement service at low cost.
