Custom protein expression
For quantitative mass spectrometry projects focusing on one or only a few proteins, purified stable isotope-labelled (SIL) proteins are the gold standard. The biochemical properties of the SIL proteins are similar to those of the respective target protein, making the SIL protein ideally suited for absolute protein quantification using liquid chromatography-mass spectrometry (LC-MS).
The SIL proteins can be added at early time points during sample preparation, providing highly accurate quantification results.
We offer
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Production of recombinant full length proteins in E. coli for application as reference standard in targeted mass spectrometry
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Isotopic labelling of your choice or unlabelled proteins
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Different grades of purity (crude extract, IMAC purified, high purity)
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Quality control with LC-MS/MS (labelling, purity)
Our highly skilled professionals have years of experience producing recombinant proteins in E. coli at various scales. Our service includes protein production from gene to protein, starting with small scale expression (ng to µg scale) up to production of several grams of protein* (large scale).
*The production of reference standards for mass spectrometry requires correct sequence and length of the proteins, but not correct folding and/or activity. Thus, unless stated otherwise for certain proteins, the purified proteins will be not checked for correct three-dimensional folding and/or activity.
