Tropoelastin
Lyophilized, human, recombinant protein. For research use only.
Product information
Tropoelastin is delivered as Lyophilized powder Package sizes 1 mg, 2.5 mg, individual packaging Type Recombinant protein Source Bacteria Endotoxin (LAL-test) <10 EU/ml (for orders of 1-10 mg); <1000 EU/ml for larger orders (> 100 mg) Sterilization method Filtration Storage conditions Powder: -80°C – -20°C, stable for at least 24 months
Solution: 2-8°C for several weeks (or store aliquots at -80°C – -20°C for longer storage times)Other HIS-tagged Solubility/Reconstitution Powder is soluble in: 0.5% acetic acid, 0.1% formic acid, 0.1% TFA
Powder can be reconstituted in phosphate buffer (50 mM Na-phosphate pH 7.0) or PBS, leaving some precipitateApplications
Tropoelastin’s flexibility and cell adhesion and signaling properties make it a highly versatile material for tissue engineering. Furthermore, it has been shown that recombinant tropoelastin supports cell attachment and enhances proliferation of various cell types. The most common applications are as:
Surface coating for cell culture
Tropoelastin is typically used as surface coating for cell culture, providing an attachment framework for the adhesion and growth of a variety of cell types [PubMed]. The authors incubated tissue culture-treated plates with full-length tropoelastin or truncations (1.5 mg/ml in PBS) overnight at 4 °C. Plates were washed twice with PBS to remove excess tropoelastin immediately before use. The Tropoelastin solution could be reused multiple times without any detectable difference in performance.
Hydrogels
Hydrogels are water-saturated polymer structures buildt by crosslinking a hydrophilic monomer with other co-monomers. Tropoelastin-containing hydrogels have been shown to support cellular growth and proliferation of different cell types [PubMed].
Fibers
Electrospun tropoelastin can act as microfiber scaffolds for adipose-derived stem cell (ADSC) delivery vehicles for treatment of wound healing. Thereby tropoelastin is able to accelerate wound healing as shown by [PubMed].
Other possible applications are summarized here: [PubMed].
About Tropoelastin
Tropoelastin is a highly flexible protein with alternating hydrophilic and hydrophobic domains. In humans, there are 13 isoforms known which are all derived from the ELN gene by alternative splicing. The molecular weight of these individual isoforms ranges from approx. 50 – 70 kDa.
Tropoelastin functions as the soluble precursor molecule of elastin. After its expression in the cell it is secreted and forms aggregates that become cross-linked and incorporated into growing elastic fibers.
With a half-life of >70 years elastin is a highly stable protein. Moreover, it is a fundamental component of the extracellular matrix (ECM) of tissues whose function requires flexibility (e.g. blood vessels, heart, lung or skin). Elastin provides elasticity and strength and enables these tissues to return to their original shape after being stretched. Besides that, elastin is also involved in wound healing.
Due to its immediate turnover into elastic fibers, native tropoelastin is not available in the cell and therefore cannot be purified from tissues. Using recombinant methods, we are able to provide human recombinant tropoelastin as sterile, lyophilized protein of high purity for research applications.
Technical information
Shipping information
Our prices are net prices, i.e. they do not contain value added taxes or customs fees. These taxes have to be paid by the customer.
Ordered products are usually shipped 1-3 business days after receiving the order.
Delivery costs depend on packaging and destination and have to be paid by the customer.