RePLiCal – peptide mix, digested, lyophilized
Retention time standard for HPLC instrument calibration
RePLiCal comprises of unique, easy to detect peptides with selected chemical properties.
- High number of calibrant points, covering a broad range of RTs
- Stable RTs over long analysis times, allowing the prediction of RTs for other peptides in a timely manner
- Includes two highly hydrophilic peptides for testing of column trapping performance
Product information
RePLiCal is a HPLC calibration standard that is buildt from 27 lysine-terminating calibrant peptides. The peptides do not contain methionine, tryptophan, cysteine, and N-terminal glutamine residues. Also aspartic acid-proline, asparagine-proline and asparagine-glycine motifs are absent. It can be applied to calibration of LC gradients and nano uHPLC instruments using trap-elute configurations, real-time scheduling of selected reaction monitoring (SRM) experiments and recalibration of the m/z scale of mass spectrometry (MS) data post-acquisition. Find more information in the original publication.
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Shipping information
Our prices are net prices, i.e. they do not contain value added taxes or customs fees. These taxes have to be paid by the customer.
Ordered products are usually shipped 1-2 business days after receiving the order.
The delivery costs depend on destination and packaging and have to be paid by the customer.Technical information
Application:
1 µg RePLiCal or 25510 fmol (digested or undigested) equals 250 – 500 injections (50 – 100 fmol/injection).
iRTs